Assay of Staphylococcal Enterotoxin from Cheese
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چکیده
منابع مشابه
Rapid and sensitive sandwich enzyme-linked immunosorbent assay for detection of staphylococcal enterotoxin B in cheese.
A rapid and sensitive screening sandwich enzyme-linked immunosorbent assay (ELISA) was developed for the detection of staphylococcal enterotoxin B (SEB) in cheese by using a highly avid anti-SEB antibody (Ab) as the capture Ab (CAb) and as the biotinylated Ab conjugate. The glutaraldehyde fixation method for the immobilization of CAb on polystyrene dipsticks was superior to the adsorption fixat...
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A simple, rapid method, based on the Oudin single diffusion technique, is described for the quantitative assay of staphylococcal enterotoxin B. The method yields reproducible results without close control of such assay variables as temperature, antiserum dilution, and assay time, provided that the ionic strength is maintained above 0.2 n sodium chloride equivalent.
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The rapid microtiter technique was investigated as a means of facilitating the detection of staphylococcal enterotoxin B. With this technique, many samples were assayed simultaneously, and readable results were obtained in 3 hr. Other advantages of this method, in addition to speed, were the small quantity of reactants used, ease of reading, and reproducibility.
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A simple homogeneous enzyme immune assay was developed for detection of staphylococcal enterotoxin B by a conjugate of beta-amylase coupled with the enterotoxin.
متن کاملDetermination of staphylococcal enterotoxin A in cheddar cheese produced without starter activity.
Three variants of the chloramine-T radioiodination method were used to iodinate staphylococcal enterotoxin A with 125I. Only one method consistently produced usable labels for radioimmunoassay. The iodine incorporation was 55 to 76%; the specific activity was 3.5 to 5.5 muCi/microgram of enterotoxin, and the label was extremely stable on storage at -20 degrees C. Determinations of the enterotox...
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ژورنال
عنوان ژورنال: Journal of Dairy Science
سال: 1965
ISSN: 0022-0302
DOI: 10.3168/jds.s0022-0302(65)88246-7